NGS Disease Research Panels
ClearSeq AMLHS
Access Targets for Acute Myeloid Leukemia (AML) Research
Now with Molecular Barcodes!
Overview
Benefits
Acute myeloid leukemia (AML) is the most common myeloid neoplasm affecting
adults and the role of chromosomal structural variations in its molecular pathogenesis
have been well-established.1 In recent years, next generation sequencing has led to
a revolution in the study of hematological malignancies and shown that mutations
and indels play an essential part in the pathogenesis of AML.
Expert-Defined Content
– Designed in collaboration with
Dr. Robert Ohgami and Dr. Daniel
Arber at Stanford University
– Targets 20 key genes frequently
mutated in AML
The ClearSeq AML was designed in collaboration with Dr. Robert Ohgami and Dr.
Daniel Arber at Stanford University. It targets 48 selected exons in 20 genes found
to be commonly mutated in AML and to be associated with myelodysplastic
syndromes and myeloproliferative neoplasms.
Premium Performance You Can Trust
– Provides 99.9% design coverage
of targeted exons
The identification of rare variants, such as those in heterogeneous tumor samples,
can be practically limited by the error rate associated with the sequencing process
itself.2 The ClearSeq AMLHS is an enhanced, high-sensitivity version of ClearSeq
AML that addresses this limitation. Molecular barcodes have been incorporated to
increase the accuracy of variant calls by allowing users to identify and remove false
positive calls due to PCR and sequencing artifacts. It also has greater uniformity of
coverage due to the addition of more overlapping probes in target genes, increasing
the coverage of targeted regions to 99.4% at 20x read depth (Figure 1). Finally, the
product supports DNA input of 50 ng compared with 200 ng input DNA required by
the original kit.
– Confidently discern variants of very
low frequency, down to 0.5%
– Library-prep free target enrichment
in six hours
Comprehensive Workflow Solution
– Get from raw data to mutation
report in 3 simple steps using
SureCall analysis software
– Obtain all reagents for sample
preparation, QC and automation
tools from one trusted partner
More Accurate and Sensitive Variant Calling
The ClearSeq AMLHS incorporates HaloPlexHS technology, which is an amplicon-
based, NGS sample preparation method that employs molecular barcodes to
enable detection of variant allele frequencies at below 1%.3 Molecular barcodes
ꢀene ꢁist ꢂtargeted eꢃonsꢄ
ꢆꢇꢈꢁ1 12
ꢉꢏꢐ2 8, 1ꢀ, 18
Fꢁꢊꢋ 14, 20
Mꢅꢁ
10
ꢇFꢋB1 13–15, 1ꢀ
ꢇRꢇF2 1
ꢍꢇFꢋR 14, 1ꢀ
NꢅM1 11
NRꢆꢇ 2, 3
RUNꢈ1 3, 4, 8
ꢇꢉꢊBꢅ1 3
www.agilent.com/genomics/AML
ꢍBꢁ
ꢍꢉBꢅꢆ
8, 9
1
ꢑꢎꢐ1
ꢑꢎꢐ2
4
4
ꢊꢉꢊ2 3, 9, 10, 11
ꢊꢅꢌꢋ 5–8
U2ꢆF1 2, 6
ꢎNMꢊꢋꢆ 4, 8, 13, 15, 16, 18
ꢒꢆꢓ2 12, 14
19, 20, 22, 23
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