Datasheet > G7555B

G7555B

更新时间： 2024-10-30 01:23:43

品牌	Logo	应用领域
惠普 - HP		/
页数	文件大小	规格书
2页	210K
描述
SureGuide CRISPR Libraries

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中文翻译

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数据手册

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G7555B 数据手册

SureGuide CRISPR Libraries

GeCKOv2 Catalog Libraries

Beneﬁts

SureGuide CRISPR Libraries

SureGuide Catalog CRISPR Libraries

Agilent’s pooled libraries come in a variety of formats designed to suit your unique

experimental requirements. Pooled libraries require both uniform representation

and high fidelity to reduce screening, improve analysis and speed your time to

results. All of our libraries, whether standard, genome-wide content or fully

customized pools, are synthesized on our industry-leading DNA manufacturing

system, so you can be sure of getting the same quality and consistency out

of every assay. Our CRISPR libraries are also one of the few licensed solutions

for conducting research with pooled guide libraries, making sure that you have

not only the best libraries available but the freedom to use them for the research

you’ve designed.

–

Exome-wide knock-down screening -

Validated guides targeting all known

genes

–

Highest quality, sequence-veriﬁed

GeCKOv2 libraries

Lentiviral, ready-to-package plasmid

libraries

Human and mouse libraries - Common

model organisms/cell lines for genome-

wide screening

Catalog Ready-to-Package Plasmid Library

Agilent’s catalog CRISPR guide libraries target all exonic sequences in the human

and mouse genome and are ideal for genome-wide functional screening. These

libraries contain content developed by and licensed by a well known research

institute. In comparison to other GeCKO libraries, Agilent’s GeCKO libraries

contain fewer missed guides and more uniform representation across the

library (Table 1). Both the mouse and human GeCKO libraries come as plasmids,

with a puromycin selection marker and a GFP reporter, that can be used to

generate high-titers of lentiviral particles.

–

Full ﬂexibility to develop your research

applications

Integration of SureGuide CRISPR library in Genomics Assay Workflow

OLS Library

PCR amplify

Clone into

viral vector

Transform

E. coli host

Isolate

plasmid DNA

Transfect

packaging line

Isolate viral

supernatant

Catalog Ready-to-Package Library

Custom Ready-to-Clone Library

Custom Ready-to-Amplify Library

Figure 1. Agilent offers CRISPR libraries in a variety of formats, from plasmid libraries to unamplified oligo pools.

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